A family of solutions of the incompressible Navier-Stokes equations is said to present anomalous dissipation if energy dissipation due to viscosity does not vanish in the limit of small viscosity. In this article we present a proof of absence of anomalous dissipation for 2D flows on the torus, with an arbitrary non-negative measure plus an integrable function as initial vorticity and square-integrable initial velocity. Our result applies to flows with forcing and provides an explicit estimate for the dissipation at small viscosity. The proof relies on a new refinement of a classical inequality due to J. Nash.